Analysis of microarrays by MALDI-TOF MS.
Identifieur interne : 000877 ( Main/Exploration ); précédent : 000876; suivant : 000878Analysis of microarrays by MALDI-TOF MS.
Auteurs : RBID : pubmed:23757366English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Lectins.
- chemical , chemistry : Lipids, Tin Compounds.
- chemical , metabolism : Transferases.
- Protein Array Analysis, Proteomics, Spectrometry, Fluorescence, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Surface Properties.
Abstract
Ligand libraries can be printed onto a sandwich composed of activated lipids embedded in a hydrophobic layer conjugated to an indium-tin oxide (ITO) surface. Arrays produced this way can be analyzed by fluorescence spectroscopy and mass spectrometry. Applications include the assignment of enzyme specificity, the profiling of glycoforms and the identification of lectins.
DOI: 10.1002/anie.201302455
PubMed: 23757366
Links toward previous steps (curation, corpus...)
Le document en format XML
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<author><name sortKey="Beloqui, Ana" uniqKey="Beloqui A">Ana Beloqui</name>
<affiliation wicri:level="1"><nlm:affiliation>CICbiomaGUNE, Biofunctional Nanomaterials Unit, Paseo Miramon 182, 20009 San Sebastian, Spain.</nlm:affiliation>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea>CICbiomaGUNE, Biofunctional Nanomaterials Unit, Paseo Miramon 182, 20009 San Sebastian</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Calvo, Javier" uniqKey="Calvo J">Javier Calvo</name>
</author>
<author><name sortKey="Serna, Sonia" uniqKey="Serna S">Sonia Serna</name>
</author>
<author><name sortKey="Yan, Shi" uniqKey="Yan S">Shi Yan</name>
</author>
<author><name sortKey="Wilson, Iain B H" uniqKey="Wilson I">Iain B H Wilson</name>
</author>
<author><name sortKey="Martin Lomas, Manuel" uniqKey="Martin Lomas M">Manuel Martin-Lomas</name>
</author>
<author><name sortKey="Reichardt, Niels Christian" uniqKey="Reichardt N">Niels Christian Reichardt</name>
</author>
</titleStmt>
<publicationStmt><date when="2013">2013</date>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Lectins (analysis)</term>
<term>Lipids (chemistry)</term>
<term>Protein Array Analysis</term>
<term>Proteomics</term>
<term>Spectrometry, Fluorescence</term>
<term>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</term>
<term>Surface Properties</term>
<term>Tin Compounds (chemistry)</term>
<term>Transferases (metabolism)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Lectins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Lipids</term>
<term>Tin Compounds</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Transferases</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Protein Array Analysis</term>
<term>Proteomics</term>
<term>Spectrometry, Fluorescence</term>
<term>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</term>
<term>Surface Properties</term>
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<front><div type="abstract" xml:lang="en">Ligand libraries can be printed onto a sandwich composed of activated lipids embedded in a hydrophobic layer conjugated to an indium-tin oxide (ITO) surface. Arrays produced this way can be analyzed by fluorescence spectroscopy and mass spectrometry. Applications include the assignment of enzyme specificity, the profiling of glycoforms and the identification of lectins.</div>
</front>
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<pubmed><MedlineCitation Owner="NLM" Status="MEDLINE"><PMID Version="1">23757366</PMID>
<DateCreated><Year>2013</Year>
<Month>07</Month>
<Day>11</Day>
</DateCreated>
<DateCompleted><Year>2014</Year>
<Month>02</Month>
<Day>07</Day>
</DateCompleted>
<Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1521-3773</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>52</Volume>
<Issue>29</Issue>
<PubDate><Year>2013</Year>
<Month>Jul</Month>
<Day>15</Day>
</PubDate>
</JournalIssue>
<Title>Angewandte Chemie (International ed. in English)</Title>
<ISOAbbreviation>Angew. Chem. Int. Ed. Engl.</ISOAbbreviation>
</Journal>
<ArticleTitle>Analysis of microarrays by MALDI-TOF MS.</ArticleTitle>
<Pagination><MedlinePgn>7477-81</MedlinePgn>
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<ELocationID EIdType="doi" ValidYN="Y">10.1002/anie.201302455</ELocationID>
<Abstract><AbstractText>Ligand libraries can be printed onto a sandwich composed of activated lipids embedded in a hydrophobic layer conjugated to an indium-tin oxide (ITO) surface. Arrays produced this way can be analyzed by fluorescence spectroscopy and mass spectrometry. Applications include the assignment of enzyme specificity, the profiling of glycoforms and the identification of lectins.</AbstractText>
<CopyrightInformation>Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.</CopyrightInformation>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Beloqui</LastName>
<ForeName>Ana</ForeName>
<Initials>A</Initials>
<Affiliation>CICbiomaGUNE, Biofunctional Nanomaterials Unit, Paseo Miramon 182, 20009 San Sebastian, Spain.</Affiliation>
</Author>
<Author ValidYN="Y"><LastName>Calvo</LastName>
<ForeName>Javier</ForeName>
<Initials>J</Initials>
</Author>
<Author ValidYN="Y"><LastName>Serna</LastName>
<ForeName>Sonia</ForeName>
<Initials>S</Initials>
</Author>
<Author ValidYN="Y"><LastName>Yan</LastName>
<ForeName>Shi</ForeName>
<Initials>S</Initials>
</Author>
<Author ValidYN="Y"><LastName>Wilson</LastName>
<ForeName>Iain B H</ForeName>
<Initials>IB</Initials>
</Author>
<Author ValidYN="Y"><LastName>Martin-Lomas</LastName>
<ForeName>Manuel</ForeName>
<Initials>M</Initials>
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<Author ValidYN="Y"><LastName>Reichardt</LastName>
<ForeName>Niels Christian</ForeName>
<Initials>NC</Initials>
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<Language>eng</Language>
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<PublicationType>Research Support, Non-U.S. Gov't</PublicationType>
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<ArticleDate DateType="Electronic"><Year>2013</Year>
<Month>06</Month>
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<MedlineJournalInfo><Country>Germany</Country>
<MedlineTA>Angew Chem Int Ed Engl</MedlineTA>
<NlmUniqueID>0370543</NlmUniqueID>
<ISSNLinking>1433-7851</ISSNLinking>
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<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance>Lectins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance>Lipids</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance>Tin Compounds</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>71243-84-0</RegistryNumber>
<NameOfSubstance>indium tin oxide</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>EC 2.-</RegistryNumber>
<NameOfSubstance>Transferases</NameOfSubstance>
</Chemical>
</ChemicalList>
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<MeshHeadingList><MeshHeading><DescriptorName MajorTopicYN="N">Lectins</DescriptorName>
<QualifierName MajorTopicYN="N">analysis</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName MajorTopicYN="N">Lipids</DescriptorName>
<QualifierName MajorTopicYN="N">chemistry</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName MajorTopicYN="N">Protein Array Analysis</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName MajorTopicYN="N">Proteomics</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName MajorTopicYN="N">Spectrometry, Fluorescence</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName MajorTopicYN="Y">Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName MajorTopicYN="N">Surface Properties</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName MajorTopicYN="N">Tin Compounds</DescriptorName>
<QualifierName MajorTopicYN="N">chemistry</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName MajorTopicYN="N">Transferases</DescriptorName>
<QualifierName MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
</MeshHeadingList>
<KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">lectins</Keyword>
<Keyword MajorTopicYN="N">mass spectrometry</Keyword>
<Keyword MajorTopicYN="N">microarrays</Keyword>
<Keyword MajorTopicYN="N">proteomics</Keyword>
<Keyword MajorTopicYN="N">transferases</Keyword>
</KeywordList>
</MedlineCitation>
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